APPLICATIONS > pDNA

Plasmid DNA purification and quantitation

Plasmid DNA (pDNA) molecules range in size from 1 to more than 1000kb. As the demand for pDNA based gene therapy and vaccines increases, large scale, cost effective, and reproducible pDNA production is required. Final products have to contain high percentage of supercoiled pDNA and removal of impurities (degradated pDNA, RNA, proteins and DNA) has to be ensured.

With all advantages which monoliths offer we were able to design efficient process for pDNA purification  as well as chromatography column based pDNA quantification and process monitoring (using CIMac pDNA analytical columns).

A011 High-Performance Monolith Chromatography of Different Forms of Plasmid DNA by CIM® DEAE Disk Using Different Gradient Slopes
P009 Plasmid DNA Purification using Novel HIC (C4) Short Monolithic Columns
P007 Purification of Large Plasmids from E. coli

A023

 In-Process Control of pDNA Production on CIMacTM pDNA Analytical Column