CIM® Protein A-8f mL HLD (High Ligand Density) Tube Monolithic Column
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Cat. No.: 437.1002 |
General Description:
The CIM® Protein A HLD-8f high throughput Tube Monolithic Column consists of a unique polymeric monolith encased in a specially designed housing. This column is used for extremely fast, highly efficient purification of immunoglobulin G. Protein A binds to Fc portion of antibodies (IgG class) without affecting antigen binding. The design of the column allows the mobile phase to run in a radial direction from the outer to the inner surface. In this way, resolution is preserved during scale-up while maintaining the short separation layer length. The short monolithic length and the specially engineered highly porous structure allow operations at elevated flow rates with low-pressure drops (only few bars).
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Features and Benefits:
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Extremely fast separations speed up method development and decrease costs. Speed also enables in-process control and analytics. |
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High binding capacity, even for large molecules, boost productivity even to 10-fold! |
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High flow rates can be achieved at moderate pressure drops- no need for expensive equipment (pumps) |
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Same material and design accross all sizes, enable easy scale-up from analytical, semi-prep and industrial scale. |
Dimensions and Operating Conditions:
Monolith dimensions
- Outer diameter, Do: 15.0 mm
- Inner diameter, Di: 6.5 mm
- Length, L: 56.0 mm
- Bed volume, V: 8.0 mL
Operating conditions
- Flow rates: up to 400 mL/min (uav = 1340 cm/h)
WARNING: Reversing the flow direction can seriously damage the column! - Pressure: up to 20 bar (2 Mpa)
- Temperature: 4 °C (39 °F) to 50 °C (122 °F)
- Recommended Ph:
working range 2–11
cleaning-in-place 2–13 - WARNING: Avoid prolonged use at elevated temperatures!
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