PRODUCTS > CIM® Laboratory Columns

CIM® C2 Disk Monolithic Column

Cat. No.: 210.8130

General Description:

These weak hydrophobic interaction columns are used for fast, highly efficient separations of large molecules such as proteins or DNA, as well as smaller molecules such as peptides.
 

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Features and Benefits:

  • Flow unaffected resolution.
 Extremely fast separations speed up method development and decrease costs. Speed also enables in-process control and analytics.
  • High dynamic binding capacity.
 High binding capacity, even for large molecules, boost productivity even to 10-fold!
  • High throughput at low pressure drop.
 High flow rates can be achieved at moderate pressure drops- no need for expensive equipment (pumps).
  • Same material and design accross all products.
 Same material and design accross all sizes enable easy scale-up from analytical, semi-prep to industrial scale.
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Buffer A: 0.05 M phosphate buffer
Buffer B: deionized water
Gradient: 10 s isocratic at 100 %, 18 s at 60 %
Flow rate: 4 ml/min
Detection: UV at 280 nm 		Sample: test protein solution + 1.2 M ammonium
sulfate, pH 7.0 (2 mg Mb, 3 mg CA and 5 mg STI in 1 ml of buffer A)
Inj. Volume: 20 µl
Peaks:
1. Myoglobin (Mb) and 14 s at 0 % buffer A.
2. Conalbumin (CA)
3. Soy. Trypsin Inhibitor (STI)

Dimensions and Operating Conditions:

Monolith conditions
  • inner diameter: 13.4 mm
  • thickness: 2.5 mm
  • disk volume: 0.35 ml
  • ring colour: brown
Operating conditions
  • flow rates: up to 10 ml/min (530cm/h)
  • pressure: up to 50 bar (5 MPa)
  • temperature: 4°C (39°F) to 50°C (122°F)
    Recommended Ph:
    working range 1–12
    cleaning-in-place 1–14 (e. g. 1MNaOH)

CHEMISTRIES
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